Control of membrane potential in double sucrose gap voltage clamp technique.
نویسندگان
چکیده
منابع مشابه
Limitations of the double sucrose gap voltage clamp technique in tension-voltage determinations on frog atrial muscle.
The purpose of this study was to evaluate the limitations of the double sucrose gap voltage clamp technique in the determination of tension-voltage relationships for frog atrial muscle. Tension-voltage relationships were determined under two conditions. In one case we determined both the tension response and slow inward current associated with an apparent step depolarization (step-clamp) as a f...
متن کاملVoltage clamp simulations for multifiber bundles in a double sucrose gap: cable complications.
A theoretical model is presented for voltage clamp of a bundle of cylindrical excitable cells in a double sucrose gap. The preparation in the test node is represented by a single one-dimensional cable (length/diameter ratio approximately) with standard Hodgkin-Huxley kinetics for transmembrane Na current. Imperfections of voltage control due to internal (longitudinal) resistivity and external (...
متن کاملVoltage clamp simulations for multifiber bundles in a double sucrose gap: radial vs. longitudinal resistance effects.
Voltage clamp responses of a single excitable fiber were simulated using a core conductor model including a high external resistance (Rs) in series to the fiber membrane to allow for intercellular clefts in a multifiber preparation. In terms of specific resistance, Rs was between 68 and 264 omega cm2. Internal resistivity (Ri) was taken to be zero or 200 omega cm. The aim of the study was to qu...
متن کاملAutomated voltage-clamp technique.
The voltage-clamp electrophysiology method is the gold standard for measuring the function of ion channels. In the past, this technique has had limited applicability in pharmaceutical drug discovery because of its low throughput, steep learning curve, and challenges in standardization of the experiments. Recently, new electrophysiology platforms have been developed, which are based on the use o...
متن کاملAnalysis of cardiac pacemaker potential using a "voltage clamp" technique.
VASSALEE, MARIO. Analysis of card& pacemaker potential using a %ltage clamf3 technique. Am. J. Physiol. 210(6): 1335-1341. I g66.-Diastolic depolarization was studied by means of ccvoItage clamp” technique in short mammalian Purkinje fibers. Clamping the membrane potential at the maximal diastolic value resulted in an inward current rising with a time constant of 3 set in 5.4 mM K, indicating t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Circulation Research
سال: 1977
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.40.6.603